Physiological Saline Testing - Revision history

Mike Chelen: /* Additional Materials Required */

2013-03-29T02:51:42Z

Additional Materials Required

← Older revision		Revision as of 02:51, 29 March 2013
Line 27:		Line 27:
	* glassware for measurement and storage		* glassware for measurement and storage
	* pH strips are do-able, but a pH meter would be preferred		* pH strips are do-able, but a pH meter would be preferred
	** [http://www.amazon.com/Hanna-Instruments-Educational-Measuring-Resolution/dp/B0085WO0GU/ref=zg_bs_393271011_7 buy] or [http://www.66pacific.com/ph/simplest_ph.aspx build]?		** [http://www.amazon.com/Hanna-Instruments-Educational-Measuring-Resolution/dp/B0085WO0GU/ref=zg_bs_393271011_7 buy], [https://www.sparkfun.com/products/10972 buy], or [http://www.66pacific.com/ph/simplest_ph.aspx build]?

	==== Storage Requirements ====		==== Storage Requirements ====

Mike Chelen: /* Additional Materials Required */

2013-03-29T02:47:23Z

Additional Materials Required

← Older revision		Revision as of 02:47, 29 March 2013
Line 27:		Line 27:
	* glassware for measurement and storage		* glassware for measurement and storage
	* pH strips are do-able, but a pH meter would be preferred		* pH strips are do-able, but a pH meter would be preferred
	** [http://www.amazon.com/Hanna-Instruments-Educational-Measuring-Resolution/dp/B0085WO0GU/ref=zg_bs_393271011_7 buy] or [http://www.66pacific.com/ph/simplest_ph.aspx build]		** [http://www.amazon.com/Hanna-Instruments-Educational-Measuring-Resolution/dp/B0085WO0GU/ref=zg_bs_393271011_7 buy] or [http://www.66pacific.com/ph/simplest_ph.aspx build]?

	==== Storage Requirements ====		==== Storage Requirements ====

Mike Chelen: /* Additional Materials Required */

2013-03-29T02:47:04Z

Additional Materials Required

← Older revision		Revision as of 02:47, 29 March 2013
Line 27:		Line 27:
	* glassware for measurement and storage		* glassware for measurement and storage
	* pH strips are do-able, but a pH meter would be preferred		* pH strips are do-able, but a pH meter would be preferred
	** [http://www.amazon.com/Hanna-Instruments-Educational-Measuring-Resolution/dp/B0085WO0GU/ref=zg_bs_393271011_7 buy] or [~~build~~ http://www.66pacific.com/ph/simplest_ph.aspx]		** [http://www.amazon.com/Hanna-Instruments-Educational-Measuring-Resolution/dp/B0085WO0GU/ref=zg_bs_393271011_7 buy] or [http://www.66pacific.com/ph/simplest_ph.aspx build]

	==== Storage Requirements ====		==== Storage Requirements ====

Mike Chelen: /* Additional Materials Required */

2013-03-29T02:46:40Z

Additional Materials Required

← Older revision		Revision as of 02:46, 29 March 2013
Line 27:		Line 27:
	* glassware for measurement and storage		* glassware for measurement and storage
	* pH strips are do-able, but a pH meter would be preferred		* pH strips are do-able, but a pH meter would be preferred
			** [http://www.amazon.com/Hanna-Instruments-Educational-Measuring-Resolution/dp/B0085WO0GU/ref=zg_bs_393271011_7 buy] or [build http://www.66pacific.com/ph/simplest_ph.aspx]

	==== Storage Requirements ====		==== Storage Requirements ====

Yar: /* Overview */

2012-11-15T00:57:57Z

Overview

← Older revision		Revision as of 00:57, 15 November 2012
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	===Phosphate Buffered Saline (PBS)===		===Phosphate Buffered Saline (PBS)===
	====Overview====		====Overview====
			There are plenty of resources out there that provide a pretty good overview of the basics of Phosphate Buffered Saline. It's safe to consider it one of the very valuable workhorses of life science labs. It's completely compatible with cell cultures and it's relatively easy to make and handle.

	==== Example Formulation Materials (1 liter) ====		==== Example Formulation Materials (1 liter) ====

Yar: /* Solutions */

2012-11-15T00:56:20Z

Solutions

← Older revision		Revision as of 00:56, 15 November 2012
Line 22:		Line 22:


	=== Additional Materials Required ===		==== Additional Materials Required ====
	* a good balance to weigh the materials on		* a good balance to weigh the materials on
	* glassware for measurement and storage		* glassware for measurement and storage
	* pH strips are do-able, but a pH meter would be preferred		* pH strips are do-able, but a pH meter would be preferred

	=== Storage Requirements ===		==== Storage Requirements ====
	If the solution isn't going to be used soon after making it, it should be stored refrigerated and sealed securely to keep it sterile. When removing it from storage, it should probably be checked for precipitation (especially if it's something like a 10X concentration).		If the solution isn't going to be used soon after making it, it should be stored refrigerated and sealed securely to keep it sterile. When removing it from storage, it should probably be checked for precipitation (especially if it's something like a 10X concentration).

	=== Possible Vendors ===		==== Possible Vendors ====

	If sourcing the materials and tools needed turns out to be a hassle there is always the possibility of purchasing the solution in liquid (concentrate or unconcentrated) or tablet forms. This may be slightly less fun but it does come with additional quality guarantees.		If sourcing the materials and tools needed turns out to be a hassle there is always the possibility of purchasing the solution in liquid (concentrate or unconcentrated) or tablet forms. This may be slightly less fun but it does come with additional quality guarantees.

Yar: /* Example Formulation */

2012-11-15T00:55:21Z

Example Formulation

← Older revision		Revision as of 00:55, 15 November 2012
Line 12:		Line 12:
	====Overview====		====Overview====

	==== Example Formulation====		==== Example Formulation Materials (1 liter) ====
	* sodium chloride		* sodium chloride (8 g)
	* potassium chloride		* potassium chloride (0.2 g)
	* sodium phosphate, dibasic		* sodium phosphate, dibasic (1.15 g)
	* potassium phosphate, monobasic		* potassium phosphate, monobasic (0.2 g)
			* distilled or double distilled water (1 L)



			There are other formulations available if, for example, we want to do a batch of 10X PBS to store and dilute as needed.


			=== Additional Materials Required ===
			* a good balance to weigh the materials on
			* glassware for measurement and storage
			* pH strips are do-able, but a pH meter would be preferred

			=== Storage Requirements ===
			If the solution isn't going to be used soon after making it, it should be stored refrigerated and sealed securely to keep it sterile. When removing it from storage, it should probably be checked for precipitation (especially if it's something like a 10X concentration).

			=== Possible Vendors ===

			If sourcing the materials and tools needed turns out to be a hassle there is always the possibility of purchasing the solution in liquid (concentrate or unconcentrated) or tablet forms. This may be slightly less fun but it does come with additional quality guarantees.

			* Sigma-Aldrich (http://www.sigmaaldrich.com/united-states.html)
			* Invitrogen (http://products.invitrogen.com/ivgn/product/18912014)
			* Amresco (http://www.amresco-inc.com/)



	[[Category:GrindDC]]		[[Category:GrindDC]]

Yar: /* Formulation */

2012-11-11T01:37:37Z

Formulation

← Older revision		Revision as of 01:37, 11 November 2012
Line 12:		Line 12:
	====Overview====		====Overview====

	====Formulation====		==== Example Formulation====
	* sodium chloride		* sodium chloride
	* potassium chloride		* potassium chloride

Yar: /* Phosphate Buffer Saline (PBS) */

2012-11-09T01:57:57Z

Phosphate Buffer Saline (PBS)

← Older revision		Revision as of 01:57, 9 November 2012
Line 9:		Line 9:
	<br>		<br>

	===Phosphate ~~Buffer~~ Saline (PBS)===		===Phosphate Buffered Saline (PBS)===
	====Overview====		====Overview====

Yar: /* Phosphate Buffer Solution (PBS) */

2012-11-09T01:55:30Z

Phosphate Buffer Solution (PBS)

← Older revision		Revision as of 01:55, 9 November 2012
Line 9:		Line 9:
	<br>		<br>

	===Phosphate Buffer ~~Solution~~ (PBS)===		===Phosphate Buffer Saline (PBS)===
			====Overview====

	====Formulation====		====Formulation====
	* sodium chloride		* sodium chloride