DC CRISPR Initiative at HacDC - Revision history

Summakor: /* Sample Inventory */ asdf

2018-01-27T02:08:30Z

Sample Inventory: asdf

← Older revision		Revision as of 02:08, 27 January 2018
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	== Sample Inventory ==		== Sample Inventory ==
	CRISPR Trial 8/24/2017		'''CRISPR Trial 8/24/2017
			'''
	LB-Agar Plate at 1 and 20 Hours:		LB-Agar Plate at 1 and 20 Hours:
	[[File:IMG_20170825_001628s.jpg]] [[File:IMG_20170825_190805s.jpg]]		[[File:IMG_20170825_001628s.jpg]] [[File:IMG_20170825_190805s.jpg]]
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	[[File:IMG_20170825_001648s.jpg]] [[File:IMG_20170825_190912s.jpg]] [[File:IMG_20170826_032748s.jpg]] [[File:IMG_20170827_093620s.jpg]]		[[File:IMG_20170825_001648s.jpg]] [[File:IMG_20170825_190912s.jpg]] [[File:IMG_20170826_032748s.jpg]] [[File:IMG_20170827_093620s.jpg]]

	CRISPR Trial 1/12/2018		'''CRISPR Trial 1/12/2018
	Clean Agar plate after 48 hours incubation. No contamination growth observed.		'''Clean Agar plate after 48 hours incubation. No contamination growth observed.
	[[Media:CRISPR-180112-Agar_blank_48h.jpeg]]		[[Media:CRISPR-180112-Agar_blank_48h.jpeg]]
	Clean Strep-Kan plate after 48 hours incubation. No contamination growth observed.		Clean Strep-Kan plate after 48 hours incubation. No contamination growth observed.

Summakor: /* Sample Inventory */ 123

2018-01-27T02:07:38Z

Sample Inventory: 123

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	StrepKan Plate at 1, 20, 28 and 57 Hours:		StrepKan Plate at 1, 20, 28 and 57 Hours:
	[[File:IMG_20170825_001648s.jpg]] [[File:IMG_20170825_190912s.jpg]] [[File:IMG_20170826_032748s.jpg]] [[File:IMG_20170827_093620s.jpg]]		[[File:IMG_20170825_001648s.jpg]] [[File:IMG_20170825_190912s.jpg]] [[File:IMG_20170826_032748s.jpg]] [[File:IMG_20170827_093620s.jpg]]

			CRISPR Trial 1/12/2018
			Clean Agar plate after 48 hours incubation. No contamination growth observed.
			[[Media:CRISPR-180112-Agar_blank_48h.jpeg]]
			Clean Strep-Kan plate after 48 hours incubation. No contamination growth observed.
			[[Media:CRISPR-180112-StrepKan_blank_48h.jpeg]]
			Agar plate with the post-protocol bacteria incubated 48 hours (control sample): much growth (overgrown).
			[[Media:CRISPR-180112-Agar_Bact_48h.jpeg]]
			Strep-Kan plate with the post-protocol bacteria incubation 48 hours (CRISPR result): no growth.
			[[Media:CRISPR-180112-StrepKan_CrisprBact_48h.jpeg]]

	== News References ==		== News References ==
	* Video: bacteria evolving antibiotic resistance in 11 days. https://www.youtube.com/watch?v=yybsSqcB7mE		* Video: bacteria evolving antibiotic resistance in 11 days. https://www.youtube.com/watch?v=yybsSqcB7mE
	* Video: CRISPR Patent Controversy: https://www.youtube.com/watch?v=IboHEQumDGc		* Video: CRISPR Patent Controversy: https://www.youtube.com/watch?v=IboHEQumDGc

108.48.49.78: /* Worklog */ asdf

2018-01-27T01:48:42Z

Worklog: asdf

← Older revision		Revision as of 01:48, 27 January 2018
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	December 11, 2017 - Nancy and Enrique.		December 11, 2017 - Nancy and Enrique.
	Contacted kit supplier to obtain fresh ingredients.		Contacted kit supplier to obtain fresh ingredients.

			January 09, 2017 - Enrique.
			Prepared fresh Agar and Strep-Kan Agar plates. Checked for contamination growth 48 hours (none).

			January 12, 2017 - Nancy, Enrique and guests Tobi and Tom.
			Trial 4.5 of the CRISPR process. Used bacteria straight from the supply rather than a colony (not available). The result after 48 hours was much growth on the Agar plate and zero growth on the Strep-Kan plate. (no modification)

	== Sample Inventory ==		== Sample Inventory ==

108.48.49.78: /* Financial Support / Sponsors */ asdf

2018-01-27T01:43:51Z

Financial Support / Sponsors: asdf

← Older revision		Revision as of 01:43, 27 January 2018
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	Nancy W - purchased Bacterial DIY CRISPR kit.		Nancy W - purchased Bacterial DIY CRISPR kit.
	Enrique C - 5/2017 - purchased Bacterial DIY CRISPR Kit Refill.		Enrique C - 5/2017 - purchased Bacterial DIY CRISPR Kit Refill.
	Nancy W - purchased Bacterial DIY CRISPR kit refill.		Nancy W - 2017 - purchased Bacterial DIY CRISPR kit refill.
			Nancy W - 2017 - purchased temperature-controlled water bath.
			Nancy W - 2017 - loaned 1600X Optical Microscope with USB camera.
			Enrique C - 2017 - purchased supplies and gram staining kit.
			The ODIN - 01/2018- provided free sample of next-gen CRISPR kit.

	==Activities and Goals==		==Activities and Goals==

Summakor: sadf

2017-12-12T02:28:43Z

sadf

← Older revision		Revision as of 02:28, 12 December 2017
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	* '''Organizers''': Enrique C. Nancy C. Wolfson, Bobby B.
	* '''Contact''': crispr@hacdc.org
	==Project Description==		==Project Description==

			* Organizers: Enrique C., Nancy C. Wolfson, Bobby B.
			* Contact: crispr@hacdc.org
	CRISPR-Cas9 is a groundbreaking new (2012 vintage) gene editing technique. While gene editing is not a new concept, previous methods were far more expensive, slow and restricted in capabilities than CRISPR. Further, whereas previous methods only successfully edited a few percent of the exposed cells, CRISPR's efficiency approaches 100%. This is particularly important for gene editing in living multi-cellular organisms. The new technique is dramatically accelerating the pace of genetic engineering since its invention in 2012.		CRISPR-Cas9 is a groundbreaking new (2012 vintage) gene editing technique. While gene editing is not a new concept, previous methods were far more expensive, slow and restricted in capabilities than CRISPR. Further, whereas previous methods only successfully edited a few percent of the exposed cells, CRISPR's efficiency approaches 100%. This is particularly important for gene editing in living multi-cellular organisms. The new technique is dramatically accelerating the pace of genetic engineering since its invention in 2012.

Summakor: /* Project Description */ asdf

2017-12-12T02:24:24Z

Project Description: asdf

← Older revision		Revision as of 02:24, 12 December 2017
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	==Project Description==		==Project Description==

	CRISPR-Cas9 is a groundbreaking new (2012 vintage) gene editing technique.		CRISPR-Cas9 is a groundbreaking new (2012 vintage) gene editing technique. While gene editing is not a new concept, previous methods were far more expensive, slow and restricted in capabilities than CRISPR. Further, whereas previous methods only successfully edited a few percent of the exposed cells, CRISPR's efficiency approaches 100%. This is particularly important for gene editing in living multi-cellular organisms. The new technique is dramatically accelerating the pace of genetic engineering since its invention in 2012.
	~~Organizers: Enrique C. Nancy C. Wolfson, Bobby B.~~
	~~Contact: crispr@hacdc.org~~

	While gene editing is not a new concept, previous methods were far more expensive, slow and restricted in capabilities than CRISPR. Further, whereas previous methods only successfully edited a few percent of the exposed cells, CRISPR's efficiency approaches 100%. This is particularly important for gene editing in living multi-cellular organisms. The new technique is dramatically accelerating the pace of genetic engineering since its invention in 2012.

	CRISPR is an acronym that describes a genetic curiosity observed several decades ago: Clusters of Regularly Interspaced Short Palindromic Repeats. A few years go it was recognized that these odd DNA sequences in bacteria are deactivated virus DNA and make up a kind of Virus Definition Database. Finally, it was discovered that a protein exists which goes around scanning bacteria DNA searching for matches to virus DNA sequences and, when a match is found, cuts the gene in the bacteria DNA very precisely and efficiently. This protein was termed Cas9. In effect, bacteria and other organisms already have an excellent built-in gene-editing mechanism, and scientists have since learned to hijack that mechanism using engineered RNA sequences that direct the Cas9 protein to cut DNA in any desired location in the genome.		CRISPR is an acronym that describes a genetic curiosity observed several decades ago: Clusters of Regularly Interspaced Short Palindromic Repeats. A few years go it was recognized that these odd DNA sequences in bacteria are deactivated virus DNA and make up a kind of Virus Definition Database. Finally, it was discovered that a protein exists which goes around scanning bacteria DNA searching for matches to virus DNA sequences and, when a match is found, cuts the gene in the bacteria DNA very precisely and efficiently. This protein was termed Cas9. In effect, bacteria and other organisms already have an excellent built-in gene-editing mechanism, and scientists have since learned to hijack that mechanism using engineered RNA sequences that direct the Cas9 protein to cut DNA in any desired location in the genome.

Summakor: /* Project Description */ qwsd

2017-12-12T02:23:42Z

Project Description: qwsd

← Older revision		Revision as of 02:23, 12 December 2017
Line 3:		Line 3:
	==Project Description==		==Project Description==

	CRISPR-Cas9 is a groundbreaking new (2012 vintage) gene editing technique. While gene editing is not a new concept, previous methods were far more expensive, slow and restricted in capabilities than CRISPR. Further, whereas previous methods only successfully edited a few percent of the exposed cells, CRISPR's efficiency approaches 100%. This is particularly important for gene editing in living multi-cellular organisms. The new technique is dramatically accelerating the pace of genetic engineering since its invention in 2012.		CRISPR-Cas9 is a groundbreaking new (2012 vintage) gene editing technique.
			Organizers: Enrique C. Nancy C. Wolfson, Bobby B.
			Contact: crispr@hacdc.org

			While gene editing is not a new concept, previous methods were far more expensive, slow and restricted in capabilities than CRISPR. Further, whereas previous methods only successfully edited a few percent of the exposed cells, CRISPR's efficiency approaches 100%. This is particularly important for gene editing in living multi-cellular organisms. The new technique is dramatically accelerating the pace of genetic engineering since its invention in 2012.

	CRISPR is an acronym that describes a genetic curiosity observed several decades ago: Clusters of Regularly Interspaced Short Palindromic Repeats. A few years go it was recognized that these odd DNA sequences in bacteria are deactivated virus DNA and make up a kind of Virus Definition Database. Finally, it was discovered that a protein exists which goes around scanning bacteria DNA searching for matches to virus DNA sequences and, when a match is found, cuts the gene in the bacteria DNA very precisely and efficiently. This protein was termed Cas9. In effect, bacteria and other organisms already have an excellent built-in gene-editing mechanism, and scientists have since learned to hijack that mechanism using engineered RNA sequences that direct the Cas9 protein to cut DNA in any desired location in the genome.		CRISPR is an acronym that describes a genetic curiosity observed several decades ago: Clusters of Regularly Interspaced Short Palindromic Repeats. A few years go it was recognized that these odd DNA sequences in bacteria are deactivated virus DNA and make up a kind of Virus Definition Database. Finally, it was discovered that a protein exists which goes around scanning bacteria DNA searching for matches to virus DNA sequences and, when a match is found, cuts the gene in the bacteria DNA very precisely and efficiently. This protein was termed Cas9. In effect, bacteria and other organisms already have an excellent built-in gene-editing mechanism, and scientists have since learned to hijack that mechanism using engineered RNA sequences that direct the Cas9 protein to cut DNA in any desired location in the genome.

Summakor: organizers & contact

2017-12-12T02:22:03Z

organizers & contact

← Older revision		Revision as of 02:22, 12 December 2017
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			* '''Organizers''': Enrique C. Nancy C. Wolfson, Bobby B.
			* '''Contact''': crispr@hacdc.org
	==Project Description==		==Project Description==

Summakor: /* Financial Support / Sponsors */ asdf

2017-12-12T01:48:58Z

Financial Support / Sponsors: asdf

← Older revision		Revision as of 01:48, 12 December 2017
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	HacDC - 8/2016 - purchased Bacterial DIY CRISPR kit.		HacDC - 8/2016 - purchased Bacterial DIY CRISPR kit.
	Enrique C - 11/2016- purchased Bacterial DIY CRISPR Kit.		Enrique C - 11/2016- purchased Bacterial DIY CRISPR Kit.
			Nancy W - purchased Bacterial DIY CRISPR kit.
	Enrique C - 5/2017 - purchased Bacterial DIY CRISPR Kit Refill.		Enrique C - 5/2017 - purchased Bacterial DIY CRISPR Kit Refill.
			Nancy W - purchased Bacterial DIY CRISPR kit refill.

	==Activities and Goals==		==Activities and Goals==

Summakor: asdf

2017-12-12T01:47:49Z

asdf

← Older revision		Revision as of 01:47, 12 December 2017
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	In the ODIN kit experiment, bacteria (E. coli HME63 strain) are modified to add resistance to the antibiotic streptomycin. The kit provides the vulnerable bacteria, the resistance gene, and growth media with and without antibiotic. The original unmodified bacteria can only grow on the plain agar media whereas bacteria with a successfully edited genome will also grow on the streptomycin-laced agar. This is similar to Wenyan Jiang, David Bikard, David Cox, Feng Zhang and Luciano Marraffini, RNA-guided editing of bacterial genomes using CRISPR-Cas systems, Nature Biotechnology 31(3), pp.233 (2013). http://zlab.mit.edu/assets/reprints/Jiang_W_Nat_Biotechnol_2013.pdf		In the ODIN kit experiment, bacteria (E. coli HME63 strain) are modified to add resistance to the antibiotic streptomycin. The kit provides the vulnerable bacteria, the resistance gene, and growth media with and without antibiotic. The original unmodified bacteria can only grow on the plain agar media whereas bacteria with a successfully edited genome will also grow on the streptomycin-laced agar. This is similar to Wenyan Jiang, David Bikard, David Cox, Feng Zhang and Luciano Marraffini, RNA-guided editing of bacterial genomes using CRISPR-Cas systems, Nature Biotechnology 31(3), pp.233 (2013). http://zlab.mit.edu/assets/reprints/Jiang_W_Nat_Biotechnol_2013.pdf

			==Financial Support / Sponsors==
			HacDC - 8/2016 - purchased Bacterial DIY CRISPR kit.
			Enrique C - 11/2016- purchased Bacterial DIY CRISPR Kit.
			Enrique C - 5/2017 - purchased Bacterial DIY CRISPR Kit Refill.


	==Activities and Goals==		==Activities and Goals==