https://old.hacdc.org/api.php?action=feedcontributions&feedformat=atom&user=Yar 2026-05-07T14:56:19Z User contributions MediaWiki 1.39.3 https://old.hacdc.org/index.php?title=Physiological_Saline_Testing&diff=8652 2012-11-15T00:57:57Z

<p>Yar: /* Overview */</p> <hr /> <div>__TOC__<br /> <br /> ==Overview==<br /> &lt;br&gt;<br /> This page is a work in progress that will describe the basic methods and plan to test device bio-proofing in saline solutions.<br /> <br /> <br /> ==Solutions==<br /> &lt;br&gt;<br /> <br /> ===Phosphate Buffered Saline (PBS)===<br /> ====Overview====<br /> There are plenty of resources out there that provide a pretty good overview of the basics of Phosphate Buffered Saline. It's safe to consider it one of the very valuable workhorses of life science labs. It's completely compatible with cell cultures and it's relatively easy to make and handle.<br /> <br /> ==== Example Formulation Materials (1 liter) ====<br /> * sodium chloride (8 g)<br /> * potassium chloride (0.2 g)<br /> * sodium phosphate, dibasic (1.15 g)<br /> * potassium phosphate, monobasic (0.2 g)<br /> * distilled or double distilled water (1 L)<br /> <br /> There are other formulations available if, for example, we want to do a batch of 10X PBS to store and dilute as needed. <br /> <br /> <br /> ==== Additional Materials Required ====<br /> * a good balance to weigh the materials on<br /> * glassware for measurement and storage<br /> * pH strips are do-able, but a pH meter would be preferred<br /> <br /> ==== Storage Requirements ==== <br /> If the solution isn't going to be used soon after making it, it should be stored refrigerated and sealed securely to keep it sterile. When removing it from storage, it should probably be checked for precipitation (especially if it's something like a 10X concentration).<br /> <br /> ==== Possible Vendors ====<br /> <br /> If sourcing the materials and tools needed turns out to be a hassle there is always the possibility of purchasing the solution in liquid (concentrate or unconcentrated) or tablet forms. This may be slightly less fun but it does come with additional quality guarantees.<br /> <br /> * Sigma-Aldrich (http://www.sigmaaldrich.com/united-states.html)<br /> * Invitrogen (http://products.invitrogen.com/ivgn/product/18912014)<br /> * Amresco (http://www.amresco-inc.com/)<br /> <br /> <br /> <br /> [[Category:GrindDC]]</div>

Yar https://old.hacdc.org/index.php?title=Physiological_Saline_Testing&diff=8651 2012-11-15T00:56:20Z

<p>Yar: /* Solutions */</p> <hr /> <div>__TOC__<br /> <br /> ==Overview==<br /> &lt;br&gt;<br /> This page is a work in progress that will describe the basic methods and plan to test device bio-proofing in saline solutions.<br /> <br /> <br /> ==Solutions==<br /> &lt;br&gt;<br /> <br /> ===Phosphate Buffered Saline (PBS)===<br /> ====Overview====<br /> <br /> ==== Example Formulation Materials (1 liter) ====<br /> * sodium chloride (8 g)<br /> * potassium chloride (0.2 g)<br /> * sodium phosphate, dibasic (1.15 g)<br /> * potassium phosphate, monobasic (0.2 g)<br /> * distilled or double distilled water (1 L)<br /> <br /> There are other formulations available if, for example, we want to do a batch of 10X PBS to store and dilute as needed. <br /> <br /> <br /> ==== Additional Materials Required ====<br /> * a good balance to weigh the materials on<br /> * glassware for measurement and storage<br /> * pH strips are do-able, but a pH meter would be preferred<br /> <br /> ==== Storage Requirements ==== <br /> If the solution isn't going to be used soon after making it, it should be stored refrigerated and sealed securely to keep it sterile. When removing it from storage, it should probably be checked for precipitation (especially if it's something like a 10X concentration).<br /> <br /> ==== Possible Vendors ====<br /> <br /> If sourcing the materials and tools needed turns out to be a hassle there is always the possibility of purchasing the solution in liquid (concentrate or unconcentrated) or tablet forms. This may be slightly less fun but it does come with additional quality guarantees.<br /> <br /> * Sigma-Aldrich (http://www.sigmaaldrich.com/united-states.html)<br /> * Invitrogen (http://products.invitrogen.com/ivgn/product/18912014)<br /> * Amresco (http://www.amresco-inc.com/)<br /> <br /> <br /> <br /> [[Category:GrindDC]]</div>

Yar https://old.hacdc.org/index.php?title=Physiological_Saline_Testing&diff=8650 2012-11-15T00:55:21Z

<p>Yar: /* Example Formulation */</p> <hr /> <div>__TOC__<br /> <br /> ==Overview==<br /> &lt;br&gt;<br /> This page is a work in progress that will describe the basic methods and plan to test device bio-proofing in saline solutions.<br /> <br /> <br /> ==Solutions==<br /> &lt;br&gt;<br /> <br /> ===Phosphate Buffered Saline (PBS)===<br /> ====Overview====<br /> <br /> ==== Example Formulation Materials (1 liter) ====<br /> * sodium chloride (8 g)<br /> * potassium chloride (0.2 g)<br /> * sodium phosphate, dibasic (1.15 g)<br /> * potassium phosphate, monobasic (0.2 g)<br /> * distilled or double distilled water (1 L)<br /> <br /> There are other formulations available if, for example, we want to do a batch of 10X PBS to store and dilute as needed. <br /> <br /> <br /> === Additional Materials Required ===<br /> * a good balance to weigh the materials on<br /> * glassware for measurement and storage<br /> * pH strips are do-able, but a pH meter would be preferred<br /> <br /> === Storage Requirements === <br /> If the solution isn't going to be used soon after making it, it should be stored refrigerated and sealed securely to keep it sterile. When removing it from storage, it should probably be checked for precipitation (especially if it's something like a 10X concentration).<br /> <br /> === Possible Vendors ===<br /> <br /> If sourcing the materials and tools needed turns out to be a hassle there is always the possibility of purchasing the solution in liquid (concentrate or unconcentrated) or tablet forms. This may be slightly less fun but it does come with additional quality guarantees.<br /> <br /> * Sigma-Aldrich (http://www.sigmaaldrich.com/united-states.html)<br /> * Invitrogen (http://products.invitrogen.com/ivgn/product/18912014)<br /> * Amresco (http://www.amresco-inc.com/)<br /> <br /> <br /> <br /> [[Category:GrindDC]]</div>

Yar https://old.hacdc.org/index.php?title=Physiological_Saline_Testing&diff=8573 2012-11-11T01:37:37Z

<p>Yar: /* Formulation */</p> <hr /> <div>__TOC__<br /> <br /> ==Overview==<br /> &lt;br&gt;<br /> This page is a work in progress that will describe the basic methods and plan to test device bio-proofing in saline solutions.<br /> <br /> <br /> ==Solutions==<br /> &lt;br&gt;<br /> <br /> ===Phosphate Buffered Saline (PBS)===<br /> ====Overview====<br /> <br /> ==== Example Formulation====<br /> * sodium chloride <br /> * potassium chloride <br /> * sodium phosphate, dibasic<br /> * potassium phosphate, monobasic<br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> [[Category:GrindDC]]</div>

Yar https://old.hacdc.org/index.php?title=Physiological_Saline_Testing&diff=8547 2012-11-09T01:57:57Z

<p>Yar: /* Phosphate Buffer Saline (PBS) */</p> <hr /> <div>__TOC__<br /> <br /> ==Overview==<br /> &lt;br&gt;<br /> This page is a work in progress that will describe the basic methods and plan to test device bio-proofing in saline solutions.<br /> <br /> <br /> ==Solutions==<br /> &lt;br&gt;<br /> <br /> ===Phosphate Buffered Saline (PBS)===<br /> ====Overview====<br /> <br /> ====Formulation====<br /> * sodium chloride <br /> * potassium chloride <br /> * sodium phosphate, dibasic<br /> * potassium phosphate, monobasic<br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> [[Category:GrindDC]]</div>

Yar https://old.hacdc.org/index.php?title=Physiological_Saline_Testing&diff=8546 2012-11-09T01:55:30Z

<p>Yar: /* Phosphate Buffer Solution (PBS) */</p> <hr /> <div>__TOC__<br /> <br /> ==Overview==<br /> &lt;br&gt;<br /> This page is a work in progress that will describe the basic methods and plan to test device bio-proofing in saline solutions.<br /> <br /> <br /> ==Solutions==<br /> &lt;br&gt;<br /> <br /> ===Phosphate Buffer Saline (PBS)===<br /> ====Overview====<br /> <br /> ====Formulation====<br /> * sodium chloride <br /> * potassium chloride <br /> * sodium phosphate, dibasic<br /> * potassium phosphate, monobasic<br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> [[Category:GrindDC]]</div>

Yar https://old.hacdc.org/index.php?title=Physiological_Saline_Testing&diff=8545 2012-11-09T01:54:01Z

<p>Yar: /* Formulation */</p> <hr /> <div>__TOC__<br /> <br /> ==Overview==<br /> &lt;br&gt;<br /> This page is a work in progress that will describe the basic methods and plan to test device bio-proofing in saline solutions.<br /> <br /> <br /> ==Solutions==<br /> &lt;br&gt;<br /> <br /> ===Phosphate Buffer Solution (PBS)===<br /> ====Formulation====<br /> * sodium chloride <br /> * potassium chloride <br /> * sodium phosphate, dibasic<br /> * potassium phosphate, monobasic<br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> [[Category:GrindDC]]</div>

Yar https://old.hacdc.org/index.php?title=Physiological_Saline_Testing&diff=8544 2012-11-09T01:53:41Z

<p>Yar: /* Phosphate Buffer Solution (PBS) */</p> <hr /> <div>__TOC__<br /> <br /> ==Overview==<br /> &lt;br&gt;<br /> This page is a work in progress that will describe the basic methods and plan to test device bio-proofing in saline solutions.<br /> <br /> <br /> ==Solutions==<br /> &lt;br&gt;<br /> <br /> ===Phosphate Buffer Solution (PBS)===<br /> ====Formulation====<br /> &lt;br&gt;<br /> * sodium chloride <br /> * potassium chloride <br /> * sodium phosphate, dibasic<br /> * potassium phosphate, monobasic<br /> &lt;br&gt;<br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> [[Category:GrindDC]]</div>

Yar https://old.hacdc.org/index.php?title=Physiological_Saline_Testing&diff=8543 2012-11-09T01:53:09Z

<p>Yar: /* Basic Formulation */</p> <hr /> <div>__TOC__<br /> <br /> ==Overview==<br /> &lt;br&gt;<br /> This page is a work in progress that will describe the basic methods and plan to test device bio-proofing in saline solutions.<br /> <br /> <br /> ==Solutions==<br /> &lt;br&gt;<br /> <br /> ===Phosphate Buffer Solution (PBS)===<br /> &lt;br&gt;<br /> * sodium chloride <br /> * potassium chloride <br /> * sodium phosphate, dibasic<br /> * potassium phosphate, monobasic<br /> &lt;br&gt;<br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> [[Category:GrindDC]]</div>

Yar https://old.hacdc.org/index.php?title=Physiological_Saline_Testing&diff=8541 2012-11-09T01:46:51Z

<p>Yar: /* Basic Formulation */</p> <hr /> <div>__TOC__<br /> <br /> ==Overview==<br /> &lt;br&gt;<br /> This page is a work in progress that will describe the basic methods and plan to test device bio-proofing in saline solutions.<br /> <br /> <br /> ==Basic Formulation==<br /> &lt;br&gt;<br /> <br /> ===Phosphate Buffer Solution (PBS)===<br /> &lt;br&gt;<br /> * sodium chloride <br /> * potassium chloride <br /> * sodium phosphate, dibasic<br /> * potassium phosphate, monobasic<br /> &lt;br&gt;<br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> [[Category:GrindDC]]</div>

Yar https://old.hacdc.org/index.php?title=Physiological_Saline_Testing&diff=8540 2012-11-09T01:46:28Z

<p>Yar: /* Basic Formulation */</p> <hr /> <div>__TOC__<br /> <br /> ==Overview==<br /> &lt;br&gt;<br /> This page is a work in progress that will describe the basic methods and plan to test device bio-proofing in saline solutions.<br /> <br /> <br /> ==Basic Formulation==<br /> <br /> ===Phosphate Buffer Solution (PBS)===<br /> &lt;br&gt;<br /> * sodium chloride <br /> * potassium chloride <br /> * sodium phosphate, dibasic<br /> * potassium phosphate, monobasic<br /> &lt;br&gt;<br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> [[Category:GrindDC]]</div>

Yar https://old.hacdc.org/index.php?title=PhysiologicalSaline&diff=8539 2012-11-09T01:45:28Z

<p>Yar: moved PhysiologicalSaline to Physiological Saline Testing: Trying to see if this makes the title more readable. I hope it doesn&#039;t break anything.</p> <hr /> <div>#REDIRECT [[Physiological Saline Testing]]</div>

Yar https://old.hacdc.org/index.php?title=Physiological_Saline_Testing&diff=8538 2012-11-09T01:45:28Z

<p>Yar: moved PhysiologicalSaline to Physiological Saline Testing: Trying to see if this makes the title more readable. I hope it doesn&#039;t break anything.</p> <hr /> <div>__TOC__<br /> <br /> ==Overview==<br /> &lt;br&gt;<br /> This page is a work in progress that will describe the basic methods and plan to test device bio-proofing in saline solutions.<br /> <br /> <br /> ==Basic Formulation==<br /> &lt;br&gt;<br /> * sodium chloride <br /> * potassium chloride <br /> * sodium phosphate, dibasic<br /> * potassium phosphate, monobasic<br /> &lt;br&gt;<br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> [[Category:GrindDC]]</div>

Yar https://old.hacdc.org/index.php?title=Physiological_Saline_Testing&diff=8537 2012-11-09T01:38:31Z

<p>Yar: </p> <hr /> <div>__TOC__<br /> <br /> ==Overview==<br /> &lt;br&gt;<br /> This page is a work in progress that will describe the basic methods and plan to test device bio-proofing in saline solutions.<br /> <br /> <br /> ==Basic Formulation==<br /> &lt;br&gt;<br /> * sodium chloride <br /> * potassium chloride <br /> * sodium phosphate, dibasic<br /> * potassium phosphate, monobasic<br /> &lt;br&gt;<br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> [[Category:GrindDC]]</div>

Yar https://old.hacdc.org/index.php?title=Physiological_Saline_Testing&diff=8536 2012-11-09T01:37:54Z

<p>Yar: </p> <hr /> <div>__TOC__<br /> <br /> ==Overview==<br /> &lt;br&gt;<br /> This page is a work in progress that will describe the basic methods and plan to test device bio-proofing in saline solutions.<br /> <br /> <br /> ==Formulation==<br /> &lt;br&gt;<br /> * sodium chloride <br /> * potassium chloride <br /> * sodium phosphate, dibasic<br /> * potassium phosphate, monobasic<br /> &lt;br&gt;<br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> [[Category:GrindDC]]</div>

Yar https://old.hacdc.org/index.php?title=Physiological_Saline_Testing&diff=8535 2012-11-09T01:33:33Z

<p>Yar: /* Formulation */</p> <hr /> <div><br /> __TOC__<br /> <br /> ==Overview==<br /> &lt;br&gt;<br /> This page is a work in progress that will describe the basic methods and plan to test device bio-proofing in saline solutions.<br /> <br /> <br /> ==Formulation==<br /> &lt;br&gt;<br /> * sodium chloride <br /> * potassium chloride <br /> * sodium phosphate, dibasic<br /> * potassium phosphate, monobasic<br /> &lt;br&gt;</div>

Yar https://old.hacdc.org/index.php?title=Physiological_Saline_Testing&diff=8534 2012-11-09T01:33:15Z

<p>Yar: /* Materials */</p> <hr /> <div><br /> __TOC__<br /> <br /> ==Overview==<br /> &lt;br&gt;<br /> This page is a work in progress that will describe the basic methods and plan to test device bio-proofing in saline solutions.<br /> <br /> <br /> ==Formulation==<br /> * sodium chloride <br /> * potassium chloride <br /> * sodium phosphate, dibasic<br /> * potassium phosphate, monobasic</div>

Yar https://old.hacdc.org/index.php?title=Physiological_Saline_Testing&diff=8533 2012-11-09T01:32:37Z

<p>Yar: </p> <hr /> <div><br /> __TOC__<br /> <br /> ==Overview==<br /> &lt;br&gt;<br /> This page is a work in progress that will describe the basic methods and plan to test device bio-proofing in saline solutions.<br /> <br /> <br /> ==Formulation==<br /> ===Materials===<br /> * sodium chloride <br /> * potassium chloride <br /> * sodium phosphate, dibasic<br /> * potassium phosphate, monobasic</div>

Yar https://old.hacdc.org/index.php?title=Physiological_Saline_Testing&diff=8532 2012-11-09T01:30:31Z

<p>Yar: /* Overview */</p> <hr /> <div><br /> <br /> __TOC__<br /> <br /> ==Overview==<br /> &lt;br&gt;<br /> This page is a work in progress that will describe the basic methods and plan to test device bio-proofing in saline solutions.<br /> <br /> <br /> ==Formulation==<br /> ===Materials===<br /> * sodium chloride <br /> * potassium chloride <br /> * sodium phosphate, dibasic<br /> * potassium phosphate, monobasic</div>

Yar https://old.hacdc.org/index.php?title=Physiological_Saline_Testing&diff=8531 2012-11-09T01:16:22Z

<p>Yar: </p> <hr /> <div><br /> <br /> ==Overview==</div>

Yar https://old.hacdc.org/index.php?title=Physiological_Saline_Testing&diff=8530 2012-11-09T01:15:44Z

<p>Yar: Created page with &quot; ==Physiological Saline Testing==&quot;</p> <hr /> <div><br /> <br /> <br /> ==Physiological Saline Testing==</div>

Yar